ldh release reagent Search Results


ldh assay reagent  (Promega)
90
Promega ldh assay reagent
Silencing of TRPV3 attenuates ischemic brain injury. (A) Top panel, a schematic TRPV3 topology illustrating the deletion of exons 13 and 14 that encoding the putative pore region and adjacent transmembrane segments five and six. And the region of residues 458–474 between S1 to S2 transmembrane domain recognized by the monoclonal TRPV3 antibody (ab94582). Bottom panel, the PCR genotyping for confirmation of Trpv3 −/− with detected cDNA band at 182 bp, Trpv3 +/+ with 304 bp, and Trpv3 +/− mice carrying the two bands. (B) Attenuation of neurological deficit scores at time points of day 1, 3 and 5 after 1.5 h tMCAO injury in Trpv3 −/− mice. Two-tailed nonparametric Mann–Whitney test. Data are shown as the median with 95% CI, and n indicates the number of mice. ∗ P < 0.05. (C) Representative TTC-stained brain slices at 24 h after reperfusion from Trpv3 +/+ and Trpv3 −/− mice. The white regions indicate the infarct size, and the red regions indicate the viable tissues. Reduction of infarction volume in Trpv3 −/− mice subjected to cerebral I/R (1.5 h/24 h) injury. (D) A decrease of brain water content in Trpv3 −/− mice subjected to cerebral I/R (1.5 h/24 h) injury. (E) Top panel, a schematic drawing for primary mouse cortical neurons subjected to 30 min oxygen-glucose deprivation and 24 h reoxygenation (OGD/R) injury. The representative images for morphological changes of Trpv3 +/+ and Trpv3 −/− neurons subjected to OGD/R injury (bottom panel). Scale bar, 100 μm. The statistics of the <t>lactate</t> <t>dehydrogenase</t> <t>(LDH)</t> release (F) in LDH assay and cell viability (G) by cell counting kit-8 (CCK8) assay of primary cortical neurons subjected to OGD/R injury from Trpv3 +/+ , Trpv3 +/− and Trpv3 −/− mice. Data are expressed as means ± SEM, n indicates the number of mice. Two-way ANOVA was used and followed by Dunnett's test from GraphPad 8. ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.
Ldh Assay Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ldh+release+reagent/cytotox+96+non+radioactive+cytotoxicity+assay/pmc09136580-82-13-16
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ldh assay reagent - by Bioz Stars, 2026-09
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lactate dehydrogenase ldh assay kit  (Beyotime)
99
Beyotime lactate dehydrogenase ldh assay kit
Silencing of TRPV3 attenuates ischemic brain injury. (A) Top panel, a schematic TRPV3 topology illustrating the deletion of exons 13 and 14 that encoding the putative pore region and adjacent transmembrane segments five and six. And the region of residues 458–474 between S1 to S2 transmembrane domain recognized by the monoclonal TRPV3 antibody (ab94582). Bottom panel, the PCR genotyping for confirmation of Trpv3 −/− with detected cDNA band at 182 bp, Trpv3 +/+ with 304 bp, and Trpv3 +/− mice carrying the two bands. (B) Attenuation of neurological deficit scores at time points of day 1, 3 and 5 after 1.5 h tMCAO injury in Trpv3 −/− mice. Two-tailed nonparametric Mann–Whitney test. Data are shown as the median with 95% CI, and n indicates the number of mice. ∗ P < 0.05. (C) Representative TTC-stained brain slices at 24 h after reperfusion from Trpv3 +/+ and Trpv3 −/− mice. The white regions indicate the infarct size, and the red regions indicate the viable tissues. Reduction of infarction volume in Trpv3 −/− mice subjected to cerebral I/R (1.5 h/24 h) injury. (D) A decrease of brain water content in Trpv3 −/− mice subjected to cerebral I/R (1.5 h/24 h) injury. (E) Top panel, a schematic drawing for primary mouse cortical neurons subjected to 30 min oxygen-glucose deprivation and 24 h reoxygenation (OGD/R) injury. The representative images for morphological changes of Trpv3 +/+ and Trpv3 −/− neurons subjected to OGD/R injury (bottom panel). Scale bar, 100 μm. The statistics of the <t>lactate</t> <t>dehydrogenase</t> <t>(LDH)</t> release (F) in LDH assay and cell viability (G) by cell counting kit-8 (CCK8) assay of primary cortical neurons subjected to OGD/R injury from Trpv3 +/+ , Trpv3 +/− and Trpv3 −/− mice. Data are expressed as means ± SEM, n indicates the number of mice. Two-way ANOVA was used and followed by Dunnett's test from GraphPad 8. ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.
Lactate Dehydrogenase Ldh Assay Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ldh+release+reagent/LDH+Cytotoxicity+Assay+Kit/pmc10158155-48-7-56
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lactate dehydrogenase release assay cyto-tox one reagent  (Promega)
90
Promega lactate dehydrogenase release assay cyto-tox one reagent
Silencing of TRPV3 attenuates ischemic brain injury. (A) Top panel, a schematic TRPV3 topology illustrating the deletion of exons 13 and 14 that encoding the putative pore region and adjacent transmembrane segments five and six. And the region of residues 458–474 between S1 to S2 transmembrane domain recognized by the monoclonal TRPV3 antibody (ab94582). Bottom panel, the PCR genotyping for confirmation of Trpv3 −/− with detected cDNA band at 182 bp, Trpv3 +/+ with 304 bp, and Trpv3 +/− mice carrying the two bands. (B) Attenuation of neurological deficit scores at time points of day 1, 3 and 5 after 1.5 h tMCAO injury in Trpv3 −/− mice. Two-tailed nonparametric Mann–Whitney test. Data are shown as the median with 95% CI, and n indicates the number of mice. ∗ P < 0.05. (C) Representative TTC-stained brain slices at 24 h after reperfusion from Trpv3 +/+ and Trpv3 −/− mice. The white regions indicate the infarct size, and the red regions indicate the viable tissues. Reduction of infarction volume in Trpv3 −/− mice subjected to cerebral I/R (1.5 h/24 h) injury. (D) A decrease of brain water content in Trpv3 −/− mice subjected to cerebral I/R (1.5 h/24 h) injury. (E) Top panel, a schematic drawing for primary mouse cortical neurons subjected to 30 min oxygen-glucose deprivation and 24 h reoxygenation (OGD/R) injury. The representative images for morphological changes of Trpv3 +/+ and Trpv3 −/− neurons subjected to OGD/R injury (bottom panel). Scale bar, 100 μm. The statistics of the <t>lactate</t> <t>dehydrogenase</t> <t>(LDH)</t> release (F) in LDH assay and cell viability (G) by cell counting kit-8 (CCK8) assay of primary cortical neurons subjected to OGD/R injury from Trpv3 +/+ , Trpv3 +/− and Trpv3 −/− mice. Data are expressed as means ± SEM, n indicates the number of mice. Two-way ANOVA was used and followed by Dunnett's test from GraphPad 8. ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.
Lactate Dehydrogenase Release Assay Cyto Tox One Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lactate dehydrogenase release assay cyto-tox one reagent - by Bioz Stars, 2026-09
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cyto-tox one reagent  (Promega)
90
Promega cyto-tox one reagent
Silencing of TRPV3 attenuates ischemic brain injury. (A) Top panel, a schematic TRPV3 topology illustrating the deletion of exons 13 and 14 that encoding the putative pore region and adjacent transmembrane segments five and six. And the region of residues 458–474 between S1 to S2 transmembrane domain recognized by the monoclonal TRPV3 antibody (ab94582). Bottom panel, the PCR genotyping for confirmation of Trpv3 −/− with detected cDNA band at 182 bp, Trpv3 +/+ with 304 bp, and Trpv3 +/− mice carrying the two bands. (B) Attenuation of neurological deficit scores at time points of day 1, 3 and 5 after 1.5 h tMCAO injury in Trpv3 −/− mice. Two-tailed nonparametric Mann–Whitney test. Data are shown as the median with 95% CI, and n indicates the number of mice. ∗ P < 0.05. (C) Representative TTC-stained brain slices at 24 h after reperfusion from Trpv3 +/+ and Trpv3 −/− mice. The white regions indicate the infarct size, and the red regions indicate the viable tissues. Reduction of infarction volume in Trpv3 −/− mice subjected to cerebral I/R (1.5 h/24 h) injury. (D) A decrease of brain water content in Trpv3 −/− mice subjected to cerebral I/R (1.5 h/24 h) injury. (E) Top panel, a schematic drawing for primary mouse cortical neurons subjected to 30 min oxygen-glucose deprivation and 24 h reoxygenation (OGD/R) injury. The representative images for morphological changes of Trpv3 +/+ and Trpv3 −/− neurons subjected to OGD/R injury (bottom panel). Scale bar, 100 μm. The statistics of the <t>lactate</t> <t>dehydrogenase</t> <t>(LDH)</t> release (F) in LDH assay and cell viability (G) by cell counting kit-8 (CCK8) assay of primary cortical neurons subjected to OGD/R injury from Trpv3 +/+ , Trpv3 +/− and Trpv3 −/− mice. Data are expressed as means ± SEM, n indicates the number of mice. Two-way ANOVA was used and followed by Dunnett's test from GraphPad 8. ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.
Cyto Tox One Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cyto-tox one reagent - by Bioz Stars, 2026-09
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reagents for the ldh release test  (Promega)
90
Promega reagents for the ldh release test
Silencing of TRPV3 attenuates ischemic brain injury. (A) Top panel, a schematic TRPV3 topology illustrating the deletion of exons 13 and 14 that encoding the putative pore region and adjacent transmembrane segments five and six. And the region of residues 458–474 between S1 to S2 transmembrane domain recognized by the monoclonal TRPV3 antibody (ab94582). Bottom panel, the PCR genotyping for confirmation of Trpv3 −/− with detected cDNA band at 182 bp, Trpv3 +/+ with 304 bp, and Trpv3 +/− mice carrying the two bands. (B) Attenuation of neurological deficit scores at time points of day 1, 3 and 5 after 1.5 h tMCAO injury in Trpv3 −/− mice. Two-tailed nonparametric Mann–Whitney test. Data are shown as the median with 95% CI, and n indicates the number of mice. ∗ P < 0.05. (C) Representative TTC-stained brain slices at 24 h after reperfusion from Trpv3 +/+ and Trpv3 −/− mice. The white regions indicate the infarct size, and the red regions indicate the viable tissues. Reduction of infarction volume in Trpv3 −/− mice subjected to cerebral I/R (1.5 h/24 h) injury. (D) A decrease of brain water content in Trpv3 −/− mice subjected to cerebral I/R (1.5 h/24 h) injury. (E) Top panel, a schematic drawing for primary mouse cortical neurons subjected to 30 min oxygen-glucose deprivation and 24 h reoxygenation (OGD/R) injury. The representative images for morphological changes of Trpv3 +/+ and Trpv3 −/− neurons subjected to OGD/R injury (bottom panel). Scale bar, 100 μm. The statistics of the <t>lactate</t> <t>dehydrogenase</t> <t>(LDH)</t> release (F) in LDH assay and cell viability (G) by cell counting kit-8 (CCK8) assay of primary cortical neurons subjected to OGD/R injury from Trpv3 +/+ , Trpv3 +/− and Trpv3 −/− mice. Data are expressed as means ± SEM, n indicates the number of mice. Two-way ANOVA was used and followed by Dunnett's test from GraphPad 8. ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.
Reagents For The Ldh Release Test, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ldh+release+reagent/ldh+release+test/pmc04919080-38-4-10
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reagents for the ldh release test - by Bioz Stars, 2026-09
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ldh assay reagent  (FUJIFILM)
90
FUJIFILM ldh assay reagent
Silencing of TRPV3 attenuates ischemic brain injury. (A) Top panel, a schematic TRPV3 topology illustrating the deletion of exons 13 and 14 that encoding the putative pore region and adjacent transmembrane segments five and six. And the region of residues 458–474 between S1 to S2 transmembrane domain recognized by the monoclonal TRPV3 antibody (ab94582). Bottom panel, the PCR genotyping for confirmation of Trpv3 −/− with detected cDNA band at 182 bp, Trpv3 +/+ with 304 bp, and Trpv3 +/− mice carrying the two bands. (B) Attenuation of neurological deficit scores at time points of day 1, 3 and 5 after 1.5 h tMCAO injury in Trpv3 −/− mice. Two-tailed nonparametric Mann–Whitney test. Data are shown as the median with 95% CI, and n indicates the number of mice. ∗ P < 0.05. (C) Representative TTC-stained brain slices at 24 h after reperfusion from Trpv3 +/+ and Trpv3 −/− mice. The white regions indicate the infarct size, and the red regions indicate the viable tissues. Reduction of infarction volume in Trpv3 −/− mice subjected to cerebral I/R (1.5 h/24 h) injury. (D) A decrease of brain water content in Trpv3 −/− mice subjected to cerebral I/R (1.5 h/24 h) injury. (E) Top panel, a schematic drawing for primary mouse cortical neurons subjected to 30 min oxygen-glucose deprivation and 24 h reoxygenation (OGD/R) injury. The representative images for morphological changes of Trpv3 +/+ and Trpv3 −/− neurons subjected to OGD/R injury (bottom panel). Scale bar, 100 μm. The statistics of the <t>lactate</t> <t>dehydrogenase</t> <t>(LDH)</t> release (F) in LDH assay and cell viability (G) by cell counting kit-8 (CCK8) assay of primary cortical neurons subjected to OGD/R injury from Trpv3 +/+ , Trpv3 +/− and Trpv3 −/− mice. Data are expressed as means ± SEM, n indicates the number of mice. Two-way ANOVA was used and followed by Dunnett's test from GraphPad 8. ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.
Ldh Assay Reagent, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ldh+release+reagent/ldh+assay+kit/pm11933134-63-6-9
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ldh assay reagent - by Bioz Stars, 2026-09
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ldh assay kit  (Solarbio Inc)
90
Solarbio Inc ldh assay kit
Silencing of TRPV3 attenuates ischemic brain injury. (A) Top panel, a schematic TRPV3 topology illustrating the deletion of exons 13 and 14 that encoding the putative pore region and adjacent transmembrane segments five and six. And the region of residues 458–474 between S1 to S2 transmembrane domain recognized by the monoclonal TRPV3 antibody (ab94582). Bottom panel, the PCR genotyping for confirmation of Trpv3 −/− with detected cDNA band at 182 bp, Trpv3 +/+ with 304 bp, and Trpv3 +/− mice carrying the two bands. (B) Attenuation of neurological deficit scores at time points of day 1, 3 and 5 after 1.5 h tMCAO injury in Trpv3 −/− mice. Two-tailed nonparametric Mann–Whitney test. Data are shown as the median with 95% CI, and n indicates the number of mice. ∗ P < 0.05. (C) Representative TTC-stained brain slices at 24 h after reperfusion from Trpv3 +/+ and Trpv3 −/− mice. The white regions indicate the infarct size, and the red regions indicate the viable tissues. Reduction of infarction volume in Trpv3 −/− mice subjected to cerebral I/R (1.5 h/24 h) injury. (D) A decrease of brain water content in Trpv3 −/− mice subjected to cerebral I/R (1.5 h/24 h) injury. (E) Top panel, a schematic drawing for primary mouse cortical neurons subjected to 30 min oxygen-glucose deprivation and 24 h reoxygenation (OGD/R) injury. The representative images for morphological changes of Trpv3 +/+ and Trpv3 −/− neurons subjected to OGD/R injury (bottom panel). Scale bar, 100 μm. The statistics of the <t>lactate</t> <t>dehydrogenase</t> <t>(LDH)</t> release (F) in LDH assay and cell viability (G) by cell counting kit-8 (CCK8) assay of primary cortical neurons subjected to OGD/R injury from Trpv3 +/+ , Trpv3 +/− and Trpv3 −/− mice. Data are expressed as means ± SEM, n indicates the number of mice. Two-way ANOVA was used and followed by Dunnett's test from GraphPad 8. ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.
Ldh Assay Kit, supplied by Solarbio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cytotoxicity ldh assay kit wst  (Dojindo Labs)
97
Dojindo Labs cytotoxicity ldh assay kit wst
Silencing of TRPV3 attenuates ischemic brain injury. (A) Top panel, a schematic TRPV3 topology illustrating the deletion of exons 13 and 14 that encoding the putative pore region and adjacent transmembrane segments five and six. And the region of residues 458–474 between S1 to S2 transmembrane domain recognized by the monoclonal TRPV3 antibody (ab94582). Bottom panel, the PCR genotyping for confirmation of Trpv3 −/− with detected cDNA band at 182 bp, Trpv3 +/+ with 304 bp, and Trpv3 +/− mice carrying the two bands. (B) Attenuation of neurological deficit scores at time points of day 1, 3 and 5 after 1.5 h tMCAO injury in Trpv3 −/− mice. Two-tailed nonparametric Mann–Whitney test. Data are shown as the median with 95% CI, and n indicates the number of mice. ∗ P < 0.05. (C) Representative TTC-stained brain slices at 24 h after reperfusion from Trpv3 +/+ and Trpv3 −/− mice. The white regions indicate the infarct size, and the red regions indicate the viable tissues. Reduction of infarction volume in Trpv3 −/− mice subjected to cerebral I/R (1.5 h/24 h) injury. (D) A decrease of brain water content in Trpv3 −/− mice subjected to cerebral I/R (1.5 h/24 h) injury. (E) Top panel, a schematic drawing for primary mouse cortical neurons subjected to 30 min oxygen-glucose deprivation and 24 h reoxygenation (OGD/R) injury. The representative images for morphological changes of Trpv3 +/+ and Trpv3 −/− neurons subjected to OGD/R injury (bottom panel). Scale bar, 100 μm. The statistics of the <t>lactate</t> <t>dehydrogenase</t> <t>(LDH)</t> release (F) in LDH assay and cell viability (G) by cell counting kit-8 (CCK8) assay of primary cortical neurons subjected to OGD/R injury from Trpv3 +/+ , Trpv3 +/− and Trpv3 −/− mice. Data are expressed as means ± SEM, n indicates the number of mice. Two-way ANOVA was used and followed by Dunnett's test from GraphPad 8. ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.
Cytotoxicity Ldh Assay Kit Wst, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Silencing of TRPV3 attenuates ischemic brain injury. (A) Top panel, a schematic TRPV3 topology illustrating the deletion of exons 13 and 14 that encoding the putative pore region and adjacent transmembrane segments five and six. And the region of residues 458–474 between S1 to S2 transmembrane domain recognized by the monoclonal TRPV3 antibody (ab94582). Bottom panel, the PCR genotyping for confirmation of Trpv3 −/− with detected cDNA band at 182 bp, Trpv3 +/+ with 304 bp, and Trpv3 +/− mice carrying the two bands. (B) Attenuation of neurological deficit scores at time points of day 1, 3 and 5 after 1.5 h tMCAO injury in Trpv3 −/− mice. Two-tailed nonparametric Mann–Whitney test. Data are shown as the median with 95% CI, and n indicates the number of mice. ∗ P < 0.05. (C) Representative TTC-stained brain slices at 24 h after reperfusion from Trpv3 +/+ and Trpv3 −/− mice. The white regions indicate the infarct size, and the red regions indicate the viable tissues. Reduction of infarction volume in Trpv3 −/− mice subjected to cerebral I/R (1.5 h/24 h) injury. (D) A decrease of brain water content in Trpv3 −/− mice subjected to cerebral I/R (1.5 h/24 h) injury. (E) Top panel, a schematic drawing for primary mouse cortical neurons subjected to 30 min oxygen-glucose deprivation and 24 h reoxygenation (OGD/R) injury. The representative images for morphological changes of Trpv3 +/+ and Trpv3 −/− neurons subjected to OGD/R injury (bottom panel). Scale bar, 100 μm. The statistics of the lactate dehydrogenase (LDH) release (F) in LDH assay and cell viability (G) by cell counting kit-8 (CCK8) assay of primary cortical neurons subjected to OGD/R injury from Trpv3 +/+ , Trpv3 +/− and Trpv3 −/− mice. Data are expressed as means ± SEM, n indicates the number of mice. Two-way ANOVA was used and followed by Dunnett's test from GraphPad 8. ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.

Journal: Acta Pharmaceutica Sinica. B

Article Title: Inhibition of intracellular proton-sensitive Ca 2+ -permeable TRPV3 channels protects against ischemic brain injury

doi: 10.1016/j.apsb.2022.01.001

Figure Lengend Snippet: Silencing of TRPV3 attenuates ischemic brain injury. (A) Top panel, a schematic TRPV3 topology illustrating the deletion of exons 13 and 14 that encoding the putative pore region and adjacent transmembrane segments five and six. And the region of residues 458–474 between S1 to S2 transmembrane domain recognized by the monoclonal TRPV3 antibody (ab94582). Bottom panel, the PCR genotyping for confirmation of Trpv3 −/− with detected cDNA band at 182 bp, Trpv3 +/+ with 304 bp, and Trpv3 +/− mice carrying the two bands. (B) Attenuation of neurological deficit scores at time points of day 1, 3 and 5 after 1.5 h tMCAO injury in Trpv3 −/− mice. Two-tailed nonparametric Mann–Whitney test. Data are shown as the median with 95% CI, and n indicates the number of mice. ∗ P < 0.05. (C) Representative TTC-stained brain slices at 24 h after reperfusion from Trpv3 +/+ and Trpv3 −/− mice. The white regions indicate the infarct size, and the red regions indicate the viable tissues. Reduction of infarction volume in Trpv3 −/− mice subjected to cerebral I/R (1.5 h/24 h) injury. (D) A decrease of brain water content in Trpv3 −/− mice subjected to cerebral I/R (1.5 h/24 h) injury. (E) Top panel, a schematic drawing for primary mouse cortical neurons subjected to 30 min oxygen-glucose deprivation and 24 h reoxygenation (OGD/R) injury. The representative images for morphological changes of Trpv3 +/+ and Trpv3 −/− neurons subjected to OGD/R injury (bottom panel). Scale bar, 100 μm. The statistics of the lactate dehydrogenase (LDH) release (F) in LDH assay and cell viability (G) by cell counting kit-8 (CCK8) assay of primary cortical neurons subjected to OGD/R injury from Trpv3 +/+ , Trpv3 +/− and Trpv3 −/− mice. Data are expressed as means ± SEM, n indicates the number of mice. Two-way ANOVA was used and followed by Dunnett's test from GraphPad 8. ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.

Article Snippet: The release of lactate dehydrogenase (LDH) into the culture medium was measured using LDH assay reagent (Promega) following the instructions of the kit menu.

Techniques: Two Tailed Test, MANN-WHITNEY, Staining, Lactate Dehydrogenase Assay, Cell Counting, CCK-8 Assay